prostate tumor du 145  (ATCC)

Journal: ACS Omega

Article Title: Novel C-2 Aromatic Heterocycle-Substituted Triterpenoids Inhibit Hedgehog Signaling in GLI1 Overexpression Cancer Cells

doi: 10.1021/acsomega.4c11479

Figure Lengend Snippet: Effect of compounds 11a and 11b on the protein expression of the Gli1 transcription factor and its transcriptional targets in A549 (A) and DU145 (B) cells following 24 h of incubation. An antibody against GAPDH was used as an internal loading control.

Article Snippet: The human prostate tumor-derived cell line DU145 was obtained from ATCC (American Type Culture Collection) and cultured in RPMI 1640 (Roswell Park Memorial Institute) medium supplemented with 10% (v/v) fetal bovine serum (FBS).

Techniques: Expressing, Incubation, Control

Journal: Pharmaceutics

Article Title: The Antitumoral/Antimetastatic Action of the Flavonoid Brachydin A in Metastatic Prostate Tumor Spheroids In Vitro Is Mediated by (Parthanatos) PARP-Related Cell Death

doi: 10.3390/pharmaceutics14050963

Figure Lengend Snippet: ( A , B ) Morphology and integrity of DU145 spheroids after treatment with Brachydin A (BrA) for 0 (Day 4), 72 (Day 7), 120 (Day 9), and 168 h (Day 11). All images were obtained using the Axio Cam MRc capture system coupled to Axio LabA1 microscope, using the 10× ( A ) and 40× ( C ) objectives. ( C ) Volume (area/µm 3 ) of DU145 spheroids after 0 (Day 4), 72 (Day 7), 120 (Day 9), and 168 h (Day 11) of exposure with BrA and respective controls. ( D ) Survival fraction of DU145 cells disaggregated from spheroids treated for 72 h with BrA and their respective controls. All data points from volume/morphology represent the mean of six ( n = 6) spheroids/replicates analyzed in three biological experiments ( n = 3). * Values statistically different from the NC group at the point (date) (* p < 0.05; ** p < 0.01; *** p < 0.001; ANOVA followed by Dunnett’s post-test). NC: negative control (RPMI 1640); SC: solvent control (1% DMSO); DTX: Docetaxel (50 μM; positive control). Scale: 200 μm.

Article Snippet: The prostate tumor DU145 cell line, isolated from a metastatic brain site, was purchased from ATCC ® (American Type Culture Collection—Cat.

Techniques: Microscopy, Negative Control, Solvent, Control, Positive Control

Journal: Pharmaceutics

Article Title: The Antitumoral/Antimetastatic Action of the Flavonoid Brachydin A in Metastatic Prostate Tumor Spheroids In Vitro Is Mediated by (Parthanatos) PARP-Related Cell Death

doi: 10.3390/pharmaceutics14050963

Figure Lengend Snippet: ( A , B ) Photomicrographs from DU145 spheroids in ECM gel obtained after 0, 24, and 48 h of exposure with Brachydin A (BrA) and respective controls. All images were acquired using the Axio Cam MRc capture system coupled to Axio LabA1 microscope, using the 10× objective, and analyzed using the AxioVision 3.1 software. Scale: 200 μm. ( C , D ) Cell migration area (µm 3 ) from DU-145 spheroids after 0, 24, and 48 h exposures with BrA and their respective controls. All points/bars represent the mean ± standard deviation of the covered area from six ( n = 6) spheroids/replicates in three biological experiments ( n = 3). * Values statistically different from the NC group at the time-point (date) (** p < 0.01; *** p < 0.001; ANOVA followed by Dunnett’s post-test). NC: negative control (RPMI 1640); SC: solvent control (1% DMSO); DTX: Docetaxel (50 μM; positive control).

Article Snippet: The prostate tumor DU145 cell line, isolated from a metastatic brain site, was purchased from ATCC ® (American Type Culture Collection—Cat.

Techniques: Microscopy, Software, Migration, Standard Deviation, Negative Control, Solvent, Control, Positive Control

Journal: Pharmaceutics

Article Title: The Antitumoral/Antimetastatic Action of the Flavonoid Brachydin A in Metastatic Prostate Tumor Spheroids In Vitro Is Mediated by (Parthanatos) PARP-Related Cell Death

doi: 10.3390/pharmaceutics14050963

Figure Lengend Snippet: ( A , B ) Photomicrographs of DU145 spheroids in bovine skin gelatin after 0 and 72 h of exposure with Brachydin A (BrA) and respective controls. The images were acquired using the Axio Cam MRc image capture system coupled to an Axio LabA1 microscope, using the 10× objective, and analyzed using the AxioVision 3.1 software. Scale: 200 μm. ( C ) Percentage (%) of cell invasion (invadopodia) formed by DU145 spheroids after 24, 48, and 72 h of exposure with BrA and respective controls. The invasion area (µm 3 ) into the ECM (gelatin) was converted to % considering the area increase at 0 h. All data points represent the mean ± standard deviation of four ( n = 4) spheroids/replicates in three biological experiments ( n = 3). * Values statistically different from the NC group at that point (date) (* p < 0.05; ** p < 0.01; *** p < 0.001; ANOVA followed by Dunnett’s post-test). NC: negative control (RPMI 1640); SC: solvent control (1% DMSO); DTX: Docetaxel (50 μM; positive control). Scale: 200 μm.

Article Snippet: The prostate tumor DU145 cell line, isolated from a metastatic brain site, was purchased from ATCC ® (American Type Culture Collection—Cat.

Techniques: Microscopy, Software, Standard Deviation, Negative Control, Solvent, Control, Positive Control

Journal: Pharmaceutics

Article Title: The Antitumoral/Antimetastatic Action of the Flavonoid Brachydin A in Metastatic Prostate Tumor Spheroids In Vitro Is Mediated by (Parthanatos) PARP-Related Cell Death

doi: 10.3390/pharmaceutics14050963

Figure Lengend Snippet: Flow cytometric analysis of DU145 spheroids treated with Brachydin A (BrA) for 72 h and respective controls and stained with Annexin V-FITC/propidium iodide (PI). ( A ) Percentage (%) of viable, early apoptotic, late apoptotic, and necrotic cells in DU145 spheroids after 72 h exposure with Brachydin A (BrA). The bars represent the mean ± standard deviation of eight ( n = 8) spheroids/replicates disaggregated in three biological experiments ( n = 3). *, # Values statistically different from the NC group ( p < 0.05; two-way ANOVA followed by Dunnett’s post-test). ( B ) Representative dot-plots of DU145 spheroids treated for 72 h with BrA. Lower left quadrant (Q3): negative cells for both Annexin V-FITC and PI; lower right quadrant (Q4): cells labeled with Annexin V (early apoptotic cells); upper left quadrant (Q1): cells labeled only with PI (necrotic cells); right upper quadrant (Q2): cells labeled with Annexin V and PI (late apoptotic cells). The bars represent the mean ± standard deviation of experiments with six ( n = 6) spheroids/replicates and three biological experiments ( n = 3). Values statistically different from the NC group for * early apoptosis; # late apoptosis and % necrosis ( p < 0.05; ANOVA followed by Dunnett’s post-test). NC: negative control (RPMI 1640); SC: solvent control (1% DMSO); DTX: Docetaxel (50 μM; positive control).

Article Snippet: The prostate tumor DU145 cell line, isolated from a metastatic brain site, was purchased from ATCC ® (American Type Culture Collection—Cat.

Techniques: Staining, Standard Deviation, Labeling, Negative Control, Solvent, Control, Positive Control

Journal: Pharmaceutics

Article Title: The Antitumoral/Antimetastatic Action of the Flavonoid Brachydin A in Metastatic Prostate Tumor Spheroids In Vitro Is Mediated by (Parthanatos) PARP-Related Cell Death

doi: 10.3390/pharmaceutics14050963

Figure Lengend Snippet: ( A , C , E , G ) Representative images of the effects of Brachydin A (BrA) and controls on mitochondrial ROS (MitoSOX Red), mitochondrial membrane potential (MitoStatus), apoptosis (Caspase 3/7), and necrosis (PI) in DU145 spheroids, respectively. The photomicrographs were acquired using the In-Cell Analyzer 2200 platform, using a 4× objective, in DAPI (Hoechst 33342), Cy5 (MitoStatus), FITC (CellEvent Caspase 3/7), and Cy3 (PI) channels. Scale: 200 µm. ( B , D , F , H ) Fluorescence intensity for MitoSOX Red (TexasRed), MitoStatus Red (Cy5), Caspase 3/7 (FITC), and PI (Cy3) in DU145 spheroids after treatment with BrA and respective controls. Data were normalized to the mean fluorescence intensity of the NC group at each time/point. The bars represent the mean ± standard deviation of four ( n = 4) spheroids/replicates from three biological experiments ( n = 3). * Values statistically different from the NC group (* p < 0.05; ** p < 0.01; *** p < 0.001; ANOVA followed by Dunnett’s post-test). NC: negative control (RPMI 1640); SC: solvent control (1% DMSO); DTX: Docetaxel (50 µM; positive control).

Article Snippet: The prostate tumor DU145 cell line, isolated from a metastatic brain site, was purchased from ATCC ® (American Type Culture Collection—Cat.

Techniques: Membrane, Fluorescence, Standard Deviation, Negative Control, Solvent, Control, Positive Control

Journal: Pharmaceutics

Article Title: The Antitumoral/Antimetastatic Action of the Flavonoid Brachydin A in Metastatic Prostate Tumor Spheroids In Vitro Is Mediated by (Parthanatos) PARP-Related Cell Death

doi: 10.3390/pharmaceutics14050963

Figure Lengend Snippet: Western blotting analysis of protein expression and densitometric quantifications in DU145 spheroids treated with BrA for 24 h ( A , B ) and 72 h ( C , D ). Protein expression was normalized with α-Tubulin (24 h) and β-Actin (72 h) from the SC group. All bars are presented as mean ± standard deviation of experiments performed with ninety-six ( n = 96) spheroids/replicates and two biological experiments ( n = 2). * Statistically different values from the SC group ( p < 0.05; ANOVA followed by Dunnett’s post-test). SC: solvent control (1% DMSO).

Article Snippet: The prostate tumor DU145 cell line, isolated from a metastatic brain site, was purchased from ATCC ® (American Type Culture Collection—Cat.

Techniques: Western Blot, Expressing, Standard Deviation, Solvent, Control

Journal: Pharmaceutics

Article Title: The Antitumoral/Antimetastatic Action of the Flavonoid Brachydin A in Metastatic Prostate Tumor Spheroids In Vitro Is Mediated by (Parthanatos) PARP-Related Cell Death

doi: 10.3390/pharmaceutics14050963

Figure Lengend Snippet: A proposed model of Brachydin A (BrA) effects in mPCa (DU145) tumor spheroids. BrA initially causes mitochondrial dysfunction and DNA fragmentation by PARP overactivation. Subsequently, cleaved-PARP promotes apoptosis/necrosis mixed-effects, DNA damage, and inflammatory response, suggesting that parthanatos cell death mediates the antiproliferative, cytotoxic, and antimetastatic properties observed in DU145 spheroids.

Article Snippet: The prostate tumor DU145 cell line, isolated from a metastatic brain site, was purchased from ATCC ® (American Type Culture Collection—Cat.

Techniques:

Journal: Cancers

Article Title: Insulin-Like Growth Factor-1 Influences Prostate Cancer Cell Growth and Invasion through an Integrin α3, α5, αV, and β1 Dependent Mechanism

doi: 10.3390/cancers14020363

Figure Lengend Snippet: IGF-1R (IGF1R) and pIGF-1R (pIGF1R) expression levels in DU145, PC3, LNCaP and VCaP cells. Light gray: isotype control; dark gray: specific fluorescence. Flow cytometry curves are representative for one of three experiments.

Article Snippet: The human prostate tumor cell lines DU145, PC3, and LNCaP were obtained from DSMZ (Braunschweig, Germany).

Techniques: Expressing, Control, Fluorescence, Flow Cytometry

Journal: Cancers

Article Title: Insulin-Like Growth Factor-1 Influences Prostate Cancer Cell Growth and Invasion through an Integrin α3, α5, αV, and β1 Dependent Mechanism

doi: 10.3390/cancers14020363

Figure Lengend Snippet: DU145, PC3, LNCaP, and VCaP cell growth in response to IGF-1. DU145 and PC3 cells were grown in FBS free medium (0%), in medium containing 2% FBS (2%) or 10% FBS (10%). LNCaP and VCaP were cultivated in the presence of 10% FBS. Untreated cells served as controls. Cell number was evaluated after 24, 48, and 72 h by the MTT assay. Error bars indicate standard deviation. Experiments were repeated five times. One representative experiment is shown. * indicates p < 0.05, # indicates p < 0.01.

Article Snippet: The human prostate tumor cell lines DU145, PC3, and LNCaP were obtained from DSMZ (Braunschweig, Germany).

Techniques: MTT Assay, Standard Deviation

Journal: Cancers

Article Title: Insulin-Like Growth Factor-1 Influences Prostate Cancer Cell Growth and Invasion through an Integrin α3, α5, αV, and β1 Dependent Mechanism

doi: 10.3390/cancers14020363

Figure Lengend Snippet: ( A ) Adhesion of DU145 and PC3 cells to immobilized collagen or fibronectin after stimulation with IGF-1 for 4 or 24 h. Mean number of adherent tumor cells from five fields. * indicates significant up-regulation to untreated control, # indicates significant down-regulation to untreated control ( n = 4). ( B ) Chemotactic movement of DU145 and PC3 cells after stimulation with IGF-1 for 4 or 24 h. Controls remained untreated. Mean number of tumor cells crawling beneath the filter membrane. * indicates significant up-regulation to untreated control, # indicates significant down-regulation to untreated control ( n = 3). All values are related to untreated controls set to 100%.

Article Snippet: The human prostate tumor cell lines DU145, PC3, and LNCaP were obtained from DSMZ (Braunschweig, Germany).

Techniques: Control, Membrane

Journal: Cancers

Article Title: Insulin-Like Growth Factor-1 Influences Prostate Cancer Cell Growth and Invasion through an Integrin α3, α5, αV, and β1 Dependent Mechanism

doi: 10.3390/cancers14020363

Figure Lengend Snippet: Motile crawling of DU145 and PC3 cells treated with IGF-1 (versus controls). The upper left side shows photomicrographs of the DU145 scratch assay taken after 0 (start), 8, 10, and 12 h (related to 4 h IGF-1 pre-stimulation). The upper right side shows the results from the quantitative calculation expressed as relative wound density (4 and 24 h pre-stimulation). DU145 were grown in 2% FBS. Lower panels: Relative wound density of DU145 cells following 4 or 24 h IGF-1 pre-stimulation and cultivation in 10% FBS, and relative wound density of PC3 cells stimulated with IGF-1 for 24 h and cultured in 2 or 10% FBS. Mean values of three experiments are shown. * indicates significant up-regulation compared to untreated controls ( n = 3).

Article Snippet: The human prostate tumor cell lines DU145, PC3, and LNCaP were obtained from DSMZ (Braunschweig, Germany).

Techniques: Wound Healing Assay, Cell Culture

Journal: Cancers

Article Title: Insulin-Like Growth Factor-1 Influences Prostate Cancer Cell Growth and Invasion through an Integrin α3, α5, αV, and β1 Dependent Mechanism

doi: 10.3390/cancers14020363

Figure Lengend Snippet: Surface expression of integrin α and β subtypes on DU145, PC3, LNCaP, and VCaP cells. Counts indicate cell number; fluorescence is expressed by mean fluorescence units (MFU). One representative of three separate experiments is shown. Solid line = specific fluorescence, dotted line = isotype control.

Article Snippet: The human prostate tumor cell lines DU145, PC3, and LNCaP were obtained from DSMZ (Braunschweig, Germany).

Techniques: Expressing, Fluorescence, Control

Journal: Cancers

Article Title: Insulin-Like Growth Factor-1 Influences Prostate Cancer Cell Growth and Invasion through an Integrin α3, α5, αV, and β1 Dependent Mechanism

doi: 10.3390/cancers14020363

Figure Lengend Snippet: IGF-1 stimulated surface expression of the integrins α3, α5, αV, and β1 on DU145 and PC3 cells, evaluated after 2, 4 and 24 h. All values are related to untreated controls. MFU: Mean fluorescence units. * indicates significant difference to controls ( n = 3).

Article Snippet: The human prostate tumor cell lines DU145, PC3, and LNCaP were obtained from DSMZ (Braunschweig, Germany).

Techniques: Expressing, Fluorescence

Journal: Cancers

Article Title: Insulin-Like Growth Factor-1 Influences Prostate Cancer Cell Growth and Invasion through an Integrin α3, α5, αV, and β1 Dependent Mechanism

doi: 10.3390/cancers14020363

Figure Lengend Snippet: Left side: Protein profile of integrins α3, α5, αV, and β1. DU145 cells were either stimulated with IGF-1 for 24 h or exposed to culture medium without IGF-1 (Ctrl). One representative of three separate experiments is shown. Each protein analysis was accompanied by a β-actin loading control. One representative internal control is shown. Right side: Pixel density analysis of the protein expression level of DU145 cells stimulated with IGF. The ratio of protein intensity/β-actin intensity is expressed as percentage of controls, indicated by line at 100%. * indicates significant difference to controls.

Article Snippet: The human prostate tumor cell lines DU145, PC3, and LNCaP were obtained from DSMZ (Braunschweig, Germany).

Techniques: Control, Expressing

Journal: Cancers

Article Title: Insulin-Like Growth Factor-1 Influences Prostate Cancer Cell Growth and Invasion through an Integrin α3, α5, αV, and β1 Dependent Mechanism

doi: 10.3390/cancers14020363

Figure Lengend Snippet: Left: Protein profile of cell signaling proteins. DU145 cells were either stimulated with IGF for 24 h or received culture medium without IGF (Ctrl). One representative of three separate experiments is shown. Each protein analysis was accompanied by a β-actin loading control. One representative internal control is shown. Right: Pixel density analysis of the protein expression level of DU145 cells stimulated with IGF. The ratio of protein intensity/β-actin intensity is expressed as percentage of controls set to 100%. * indicates significant difference to controls.

Article Snippet: The human prostate tumor cell lines DU145, PC3, and LNCaP were obtained from DSMZ (Braunschweig, Germany).

Techniques: Control, Expressing

Journal: Cancers

Article Title: Insulin-Like Growth Factor-1 Influences Prostate Cancer Cell Growth and Invasion through an Integrin α3, α5, αV, and β1 Dependent Mechanism

doi: 10.3390/cancers14020363

Figure Lengend Snippet: Left: protein profile of integrins α3, α5, αV, and β1 in PC3 cells. Right: protein profile of cell signaling proteins in PC3 cells. DU145 or PC3 cells were either stimulated with IGF for 24 h or received culture medium without IGF (Ctrl). One representative of three separate experiments is shown. Each protein analysis was accompanied by a β-actin loading control. One representative internal control is shown.

Article Snippet: The human prostate tumor cell lines DU145, PC3, and LNCaP were obtained from DSMZ (Braunschweig, Germany).

Techniques: Control

Journal: Cancers

Article Title: Insulin-Like Growth Factor-1 Influences Prostate Cancer Cell Growth and Invasion through an Integrin α3, α5, αV, and β1 Dependent Mechanism

doi: 10.3390/cancers14020363

Figure Lengend Snippet: Upper panels: cell growth dynamics of DU145, PC3, LNCaP, and VCaP cells after blockade through monoclonal antibodies against α3, α5, αV, or β1. Controls remained untreated. * indicates significant difference to untreated controls ( n = 4). Lower panels: chemotactic movement of DU145 and PC3 cells after blockade through monoclonal antibodies (specific blockade against α3, α5, αV, or β1). Controls remained untreated. All values are related to the untreated controls set to 100%. * indicates significant difference ( n = 3).

Article Snippet: The human prostate tumor cell lines DU145, PC3, and LNCaP were obtained from DSMZ (Braunschweig, Germany).

Techniques: Bioprocessing

Journal: Cancers

Article Title: Insulin-Like Growth Factor-1 Influences Prostate Cancer Cell Growth and Invasion through an Integrin α3, α5, αV, and β1 Dependent Mechanism

doi: 10.3390/cancers14020363

Figure Lengend Snippet: Adhesion modulation of DU145 and PC3 through monoclonal antibodies against α3, α5, αV, or β1 (left: adhesion to collagen; right: adhesion to fibronectin). Controls remained untreated. * indicates significant difference to untreated controls ( n = 3).

Article Snippet: The human prostate tumor cell lines DU145, PC3, and LNCaP were obtained from DSMZ (Braunschweig, Germany).

Techniques: Bioprocessing

Journal: Cancers

Article Title: Insulin-Like Growth Factor-1 Influences Prostate Cancer Cell Growth and Invasion through an Integrin α3, α5, αV, and β1 Dependent Mechanism

doi: 10.3390/cancers14020363

Figure Lengend Snippet: ( A ) Protein expression level following siRNA transfection (untreated control versus scrambled siRNA versus specific siRNA). Each protein analysis was accompanied by a β-actin loading control. One representative internal control is shown. ( B ) Cell growth of DU145 treated with an integrin α3, α5, αV, or FAK specific siRNA, evaluated by the MTT-assay. One representative of three separate experiments is shown. ( C ) Chemotactic movement of DU145 cells after knocking down integrin α3, α5, or αV. All values are related to the scrambled controls set to 100%. * indicates significant difference to controls ( n = 3).

Article Snippet: The human prostate tumor cell lines DU145, PC3, and LNCaP were obtained from DSMZ (Braunschweig, Germany).

Techniques: Expressing, Transfection, Control, MTT Assay

Journal: Cancers

Article Title: Insulin-Like Growth Factor-1 Influences Prostate Cancer Cell Growth and Invasion through an Integrin α3, α5, αV, and β1 Dependent Mechanism

doi: 10.3390/cancers14020363

Figure Lengend Snippet: ( A ) DU145 cell number in response to integrin blockade. Tumor cells were grown in 2 or 10% FBS and in the presence or without IGF-1. Untreated cells served as controls. Cell number was evaluated after 24, 48, and 72 h by the MTT assay. ( B ) Chemotactic movement of DU145 cells after blockade through monoclonal antibodies (specific blockade against α3, αV, or β1) with or without IGF-1 activation. ( C ) Adhesion modulation of DU145 through integrin β1 blockade in the presence of 2 versus 10% FBS. ( B , C ) are related to the non-blocked controls set to 100%. Error bars indicate standard deviation. Experiments were repeated three times. One representative experiment is shown. * indicates p < 0.05.

Article Snippet: The human prostate tumor cell lines DU145, PC3, and LNCaP were obtained from DSMZ (Braunschweig, Germany).

Techniques: MTT Assay, Bioprocessing, Activation Assay, Standard Deviation

Journal: Cancers

Article Title: Insulin-Like Growth Factor-1 Influences Prostate Cancer Cell Growth and Invasion through an Integrin α3, α5, αV, and β1 Dependent Mechanism

doi: 10.3390/cancers14020363

Figure Lengend Snippet: ( Left ) Integrin subtype and FAK expression level in DU145 cells following treatment with Akt specific siRNA (versus untreated controls or scrambled siRNA). ( Right ) mTOR signaling in DU145 cells following defactinib treatment for 24 and 72 h. Controls remained untreated. One representative of three separate experiments is shown. Each protein analysis was accompanied by a β-actin loading control. One representative internal control is shown.

Article Snippet: The human prostate tumor cell lines DU145, PC3, and LNCaP were obtained from DSMZ (Braunschweig, Germany).

Techniques: Expressing, Control